Instruction manual pglo bacterial transformation kit

 · pGLO™ Bacterial Transformation Kit Catalog #EDU www.doorway.ru For technical support call your local Bio-Rad office, or in the U.S., call pGLO araC GFP bla ori See individual components for storage temperature. Duplication of any part of this document is permitted for classroom use only. Bio-Rad's pGLO Bacterial Transformation Kit is the classic kit for teaching the central dogma and the basics of genetic engineering. In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, which causes the. pGLO Bacterial Transformation Kit Teach the basics of genetic engineering and the roles that external and internal factors play in gene regulation. Teach core genetics concepts with a three-dimensional approach to the favorite pGLO bacterial transformation activity.

Purification Kit Instruction Manual Catalog #EDU With the pGLO Bacterial Transformation kit, you performed a genetic transformation of E. coli bacterial cells. The results of this procedure were colonies of cells that fluoresced when exposed to ultraviolet light. This is not a normal phenotype (characteristic) for www.doorway.ru Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. Prior steps for creating recombinant plasmids are described in traditional cloning basics and involve insertion of a DNA sequence of interest into a vector backbone. In transformation, the DNA (usually in the form of a plasmid) is introduced into a. Page Adapted from pGLO Bacterial Transformation Kit (catalog number EDU), Biotechnology Explorer™ instruction manual, Rev. E. Bio-Rad Laboratories, Life Science Education. BIORAD (), www.doorway.ru

Transformation Transformation •pGlo = Plasmid which contains the gene for Green Fluorescent Protein. •GFP = Green Fluorescent Protein –The gene for this protein comes from the bioluminescent jellyfish Aequorea victoria •GFP causes the jellyfish to glow in the dark. •The bacteria will be transformed with this gene and produce the GFP. Use the pGLO Bacterial Transformation Kit Instruction Manual with the pGLO Bacterial Transformation Kit (#EDU). The manual includes the Teacher's Guide, Student Manual, and graphic Quick Guide. This product is for education use only. There is a one per customer limit. The pGLO System With the pGLO transformation kit, students use a simple procedure to transform bacteria with a gene that codes for Green Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria, and GFP causes the jellyfish to fluoresce and glow in the dark.